Abstract
Background: Helicobacter pylori infection is the most prevalent in developing countries. It is an etiological agent of peptic ulcer, gastric adenocarcinoma, and mucosal-associated lymphoid tissue (MALT) lymphoma. Despite the development of different assays to confirm H. pylori infection, the diagnosis of infection is challenged by the precision of the applied assay. Hence, this study aims to assess the diagnostic accuracy of Nested PCR using a highly specific and sensitive new untested Primer pair, especially against the routinely and commonly performed chairside Rapid Urease card test. Methods: Gastric biopsy samples were collected from patients exhibiting Helicobacter pylori infection during endoscopy, and a rapid urease card test was conducted. From twenty out of those rapid urease negative samples, DNA extraction was performed, followed by amplification using two primer pairs (Pylo A and Pylo AN) synthesized from the 16s rRNA gene of Helicobacter pylori genome. Results: Twelve (60%) of the rapid urease card test-negative gastric biopsy samples tested positive with Nested PCR. Conclusions: The results indicate that the Nested PCR is more sensitive and specific than the Rapid urease test for detecting Helicobacter pylori in gastric biopsy samples. Consequently, due to its superior diagnostic accuracy, it should be consistently utilized for patients with Gastroduodenopathy to avert any potential misdiagnosis.
Keywords: – Helicobacter Pylori, Rapid Urease Test, Nested PCR.
Dr. Divya Sharma1*, Dr. Anjli Gupta2, Dr. Sanju Pannu3Dr. Divya Sharma1*, Dr. Anjli Gupta2, Dr. Sanju Pannu3, Dr. Mohmad Rizwan4, Dr. Niranjana5, Dr. Vinay Mehra6